Soule, L.D., Skrajewski-Schuler, L., Branch, S.A., McMahon, T.J., Spence, D.M. Toward translational impact of low-glucose strategies on red blood cell storage optimization. ACS Pharmacol. Transl. Sci. 2024,


Skrajewski-Schuler, L.A., Soule, L.D., Geiger, M., Spence D. UPLC-MS/MS method for quantitative determination of the advanced glycation endproducts Nε-(carboxymethyl)lysine and Nε-(carboxyethyl)lysine. Anal. Methods 202315, 6698-6705,

Hoehn, B., Soule, L., Skrajewski, L, Spence, D., Zhu, H., McMahon, T.J. Novel normoglycemic human RBC storage: post-transfusion respiratory sequelae in nude mice. Am. J. Respir. Crit. Care Med. 2023207, A1698,

Heller, A.A., Geiger, M.K., Spence, D.M. A 3D-printed multi-compartment device that enables dynamic PK/PD profiles of antibiotics. Anal. Bioanal. Chem. 2023, 415, 6135-6144,


Geiger, M., Hayter, E., Martin, R.S., Spence, D. Red blood cells in type 1 diabetes and multiple sclerosis and technologies to measure their emerging roles. J. Transl. Autoimmun. 2022, 10.1016/j.jtauto.2022.100161

Hayter, E.A., Azibere, S., Skrajewski, L.A., Soule, L.D., Spence, D.M., Martin, R.S. A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells. Anal Methods 2022, 14, 3171-3179,

Jacobs, M., Geiger, M., Summers, S., Janes, T., Boyea, R., Zinn, K., Aburashed, R., Spence,D. Interferon-β decreases the hypermetabolic state of red blood cells from patients with multiple sclerosis. ACS Chem. Neurosci. 2022,

Jacobs, M.J., Geiger, M.K., Summers, S.E., DeLuca, C.P., Zinn, K.R., Spence, D.M. Albumin glycation affects the delivery of C-peptide to the red blood cells. ACS Meas. Sci. Au. 2022, 2(3), 278-286,

Liu, Y., Hesse, L.E., Geiger, M.K., Zinn, K.R., McMahon, T.J., Chen, C., Spence, D.M. A 3D-printed transfusion platform reveals beneficial effects of normoglycemic erythrocyte storage solutions and a novel rejuvenating solution. Lab Chip. 2022, 22, 1310-1320,


Keshavarz, H., Meints, L.M., Geiger, M. K., Zinn, K.R., Spence, D.M. Specific binding of leptin to red blood cells delivers a pancreatic hormone and stimulates ATP release. Mol. Pharm. 2021, 18 (6), 2438-2447.


Geiger, M., Janes, T., Keshavarz, H., Summers, S., Pinger, C., Fletcher, D., Zinn, K., Tennakoon, M., Karunarathne, A., Spence, D.  A C-peptide complex with albumin and Zn2+ increases measurable GLUT1 levels in membranes of human red blood cells. Sci. Rep. 2020, 10 (17493), 10.1038/s41598-020-74527-6

Ghanbarpour, A., Santos, E.M., Pinger, C., Assar Z., Nasr, S.H., Vasileiou, C., Spence, D., Borhan, B., Geiger, J.H. Human cellular retinol binding protein II forms a domain-swapped trimer representing a novel fold and a new template for protein engineering. ChemBioChem 2020, 21(22), 3192-3196,

Jacobs, M.J., Pinger, C.W., Castiaux, A.D., Maloney, K.J., Spence, D.M. A novel 3D-printed centrifugal ultrafiltration method reveals in vivo glycation of human serum albumin decreases its binding affinity for zinc. Metallomics 2020, 12(7), 1036-1043,

Pinger, C., Geiger, M., & Spence, Dana.  Applications of 3D-printing for improving chemistry education. J. Chem. Edu. 2020, 97 (1), 112-117,


Castiaux, A.D., Spence, D.M., Martin, R.S. Review of 3D cell culture with analysis in microfluidic systems. Anal. Methods, 2019, 11, 4220-4232,

Castiaux, A.D., Pinger, C.W., Hayter, E.A., Bunn, M.E., Martin, R.S., Spence, D.M. PolyJet 3D-printed enclosed microfluidic channels without photocurable supports. Anal. Chem. 2019, 91(10), 6910-6917,

Ghanbarpour, A., Pinger, C., Salmani, R.E., Assar, Z., Santos, E.M., Nosrati, M., Pawlowski, K., Spence, D., Vasileiou, C., Jin, X., Borhab, B., Geiger, J.H. Engineering the hCRBPII domain-swapped dimer into a new class of protein switches. J. Am. Chem. Soc. 2019, 141(43), 17125-17132,

Heller, A.A., Spence, D.M. A rapid method for post-antibiotic bacterial susceptibility testing. PLOS One 2019,

Huang, S., Aregullin, E.O., Gosnell, J.M., Samuel, B.P., Kaley, V.R., Castiaux, A., Pinger, C., Apkinar, M.H., Chinnadurai, P., Spence, D.M., Contag, C.H., Vettukattil, J.J. SN Compr. Clin. Med. 2019, 1, 996-1000,


Castiaux, A.D., Pinger, C.W., Spence, D.M. Ultrafiltration binding analyses of glycated albumin with a 3D-printed syringe attachment. Anal. Bioanal. Chem. 2018, 410, 7565-7573,

Janes, T.M., Spence, D.M. Steroid inhibition of erythrocyte-derived ATP reduces endothelial cell production of nitric oxide in a 3D-pritned fluidic model. Anal. Methods 2018, 10, 3416-3422,

Pinger, C.W, Castiaux, A., Speed, S., Spence, D.M. Plate reader compatible 3D-printed device for teaching equilibrium dialysis binding assays. J. Chem. Educ. 2018, 95(9), 1662-1667,


Gross, B., Lockwood, S.Y., Spence, D.M. Recent advances in analytical chemistry by 3D printing. Anal. Chem. 2017, 89(1), 57-70,

Pinger, C.W., Entwistle, K.E., Bell, T.M., Liu, Y., Spence, D.M. C-peptide replacement therapy in type 1 diabetes: are we in the trough of disillusionment? Mol. BioSyst. 2017, 8, 1432-1437,

Pinger, C.W., Heller, A.A., Spence, D.M. A printed equilibrium dialysis device with integrated membranes for improved binding affinity measurements. Anal. Chem., 2017, 89(14), 7302-7306,


Chen, C., Mehl, B.T., Munshi, A.S., Townsend, A.D., Spence, D.M., Martin, R.S. 3D-printed microfluidic devices: fabrication, advantages and limitations-a mini review. Anal. Methods, 2016, 8, 6005-6012,

LaBonia, G.J., Lockwood, S.Y., Heller, A.A., Spence, D.M., Hummon, A.B. Drug penetration and metabolism in 3D cell cultures treated in a 3D printed fluidic device: assessment of irinotecan via MALDI imaging mass spectrometry. Proteomics 2016, 11-12, 1814-1821,

Lockwood, S.Y., Meisel, J.E., Monsma, F.J., Spence, D.M. A diffusion-based and dynamic 3D-printed device that enables parallel in vitro pharmacokinetic profiling of molecules. Anal. Chem. 2016, 88(3), 1864-1870,

Lockwood, S.Y., Summers, S., Eggenberger, E., Spence, D.M. An in vitro diagnostic for multiple sclerosis based on C-peptide binding to erythrocytes. EBioMedicine 2016, 11, 249-252,

Mu, R., Chen, C., Wang, Y., Spence, D.M. A quantitative, in vitro appraisal of experimental low-glucose storage solutions used for blood banking. Anal. Methods 2016, 8, 6856-6864,

Saytashev, I., Glenn R., Murashova, G.A., Osseiran, S., Spence, D., Evans, C.L., Dantus, M. Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood cells. Biomed. Opt. Express 2016, 7(9), 3449-3460,


Gross, B.C., Anderson, K.B., Meisel, J.E, McNitt, M.I, Spence, D.M. Polymer coatings in 3D-printed fluidic device channels for improved cellular adherence prior to electrical lysis. Anal. Chem. 2015, 87(12), 6335-6341,

Liu, Y., Chen, C., Summers, S., Medawala, W., Spence, D.M. C-peptide and zinc delivery to erythrocytes requires the presence of albumin: implications in diabetes explored with a 3D-printed fluidic device. Integr. Biol. 2015, 7(5), 534-543,


Chen, C., Wang, Y., Lockwood, S.Y., Spence, D.M. 3D-printed fluidic devices enable quantitative evaluation of blood components in modified storage solutions for use in transfusion medicine. Analyst 2014, 139, 3219-3226,

Erkal, J.L., Selimovic, A., Gross, B.C., Lockwood, S.Y., Walton, E.L., McNamara, S., Martin, R.S., Spence, D.M. 3D printed microfluidic devices with integrated versatile and reusable electrodes. Lab Chip, 2014, 14, 2023-2032,

Gross, B.C., Erkal, J.L., Lockwood, S.Y., Chen, C., Spence, D.M. Evaluation of 3D printing and its potential impact on biotechnology and the chemical sciences. Anal. Chem. 2014, 86(7), 3240-3253,

Lockwood, S.Y., Erkal, J.L., Spence, D.M. Endothelium-derived nitric oxide production is increased by ATP released from red blood cells incubated with hydroxyurea. Nitric Oxide 2014, 38, 1-7,

Selimovic, A., Erkal, J.L, Spence, D.M., Martin, R.S. Microfluidic device with tunable post arrays and integrated electrodes for studying cellular release. Analyst 2014, 139, 5686-5694,

Wang, Y., Giebink, A., Spence, D.M. Microfluidic evaluation of red cells collected and stored in modified processing solutions used in blood banking. Integr. Biol. 2014, 6(1), 65-75,


Anderson, K.B., Lockwood, S.Y., Martin, R.S., Spence, D.M. A 3D printed fluidic device that enables integrated features. Anal. Chem. 2013, 85(12), 5622-5626,

Anderson, K.B., Halpin, S.T., Johnson, A.S., Martin, R.S., Spence, D.M. Integration of multiple components in polystyrene-based microfluidic devices part II: cellular analysis. Analyst 2013, 138, 137-143,

Giebink, A.W., Vogel, P.A., Medawala, W., Spence, D.M. C-peptide-stimulated nitric oxide production in a cultured pulmonary artery endothelium is erythrocyte mediated and requires Zn2+. Diabetes Metab. Res. Rev. 2013, 29(1),

Johnson, A.S., Anderson, K.B., Halpin, S.T., Kirkpatrick, D.C., Spence, D.M., Martin, R.S. Integration of multiple components in polystyrene-based microfluidic devices part I: fabrication and characterization. Analyst 2013, 138, 129-136,


Anderson, K.B., Karunarathne, W., Spence, D.M. Measuring P2X1 receptor activity in washed platelets in the absence of exogenous apyrase. Anal. Methods 2012, 4, 101-105,

Giebink, A.W., Vogel, P.A., Medawala, W., Spence, D.M. C-peptide-stimulated nitric oxide production in a cultured pulmonary artery endothelium is erythrocyte mediated and requires Zn2+. Diabetes Metab. Res. Rev. 2012, 29(1), 44-52,

Spence, D.M. (2012). The effect of combined C-peptide and zinc on cellular function. In Sima, A.A.F. (Ed.). Diabetes & C-peptide: scientific and clinical aspects (pp. 17-29). Human Press.


Halpin, S.T., Anderson, K.B., Vogel, P.A., Spence, D.M. The red blood cell and nitric oxide: derived, stimulated, or both? The Open Nitric Oxide Journal 2011, 3(Suppl 1-M2), 8-15,

Vogel, P.A., Halpin, S.T., Martin, R.S., Spence, D.M. Microfluidic transendothelial electrical resistance measurement device that enables blood flow and postgrowth experiments. Anal. Chem. 2011, 83(11), 4296-4301,


Halpin, S.T., Spence, D.M. Direct plate-reader measurement of nitric oxide released from hypoxic erythrocytes flowing through a microfluidic device. Anal. Chem. 2010, 82(17), 7492-7497,

Keltner, Z., Meyer, J.A., Johnson, E.M., Palumbo, A.M., Spence, D.M., Reid, G.E. Mass spectrometric characterization and activity of zinc-activated proinsulin C-peptide and C-peptide mutants. Analyst 2010, 135, 278-288,

Letourneau, S., Hernandez, L., Faris, A.N., Spence, D.M. Evaluating the effects of estradiol on endothelial nitric oxide stimulated by erythrocyte-derived ATP using a microfluidic approach. Anal. Bioanal. Chem. 2010, 397, 3369-3375,

Raththagala, M., Karunarathne, W., Kryziniak, M., McCracken, J., Spence, D.M. Hydroxyurea stimulates the release of ATP from rabbit erythrocytes through an increase in calcium and nitric oxide production. Eur. J. Pharmacol. 2010, 645(1-3), 32-38,

Tolan, N.V., Meyer, J.A, Ku, C., Karunarathne, W., Spence, D.M. Use of the red blood cell as a simple drug target and diagnostic by manipulating and monitoring its ability to release adenosine triphosphate (ATP). Pure Appl. Chem. 2010, 82(8), 1623-1634,


D’Amico Oblak, T., Meyer, J.A., Spence, D.M. A microfluidic technique for monitoring bloodstream analytes indicative of C-peptide resistance in type 2 diabetes. Analyst, 2009, 134, 188-193,

Karunarathne, W., Ku, C., Spence, D.M. The dual nature of extracellular ATP as a concentration-dependent platelet P2X1 agonist and antagonist. Integr. Biol. 2009, 1(11-12), 655-663,

Keltner, Z., Meyer, J.A., Johnson, E.M., Palumbo, A.M., Spence, D.M., Reid, G.E. Mass spectrometric characterization and activity of zinc-activated proinsulin C-peptide and C-peptide mutants. Analyst 2009, 135, 278-288,

Meyer, J.A., Spence, D.M. A perspective on the role of metals in diabetes: past findings and possible future directions. Metallomics 2009, 1(1), 32-41,

Meyer, J.A., Subasinghe, W., Sima, A.A.F., Keltner, Z., Reid, G.E., Daleke, D., Spence, D.M. Zinc-activated C-peptide resistance to the type 2 diabetic erythrocyte is associated with hyperglycemia-induced phosphatidylserine externalization and reversed by metformin. Mol. BioSyst. 2009, 5, 11157-1162,

Medwala, W., McCahill, P., Giebink, A., Meyer, J., Ku, C., Spence, D.M. A molecular level understanding of zinc activation of C-peptide and its effects on cellular communication in the bloodstream. Rev. Diabet. Stud. 2009, 6(3), 148-158,

Tolan, N.V., Genes, L.I., Subasinghe, W., Raththagala, M., Spence, D.M. Personalized metabolic assessment of erythrocytes using microfluidic delivery to an array of luminescent wells. Anal. Chem. 2009, 81(8), 3102-3108,


Faris, A., Spence, D.M. Measuring the simultaneous effects of hypoxia and deformation on ATP release from erythrocytes. Analyst 2008, 133, 678-682,

Ku, C., D’Amico Oblak, T., Spence, D.M. Interactions between multiple cell types in parallel microfluidic channels: monitoring platelet adhesion to an endothelium in the presence of an anti-adhesion drug. Anal. Chem. 2008, 80(19), 7543-7548,

Meyer, J.A., Froelich, J.M., Reid, G.E., Karunarathne, W.K.A., Spence, D.M. Metal-activated C-peptide facilitates glucose clearance and the release of a nitric oxide stimulus via the GLUT1 transporter. Diabetologia 2008, 51, 175-182,

Subasinghe, W., Spence, D.M. Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes. Anal. Chim. Acta 2008, 2(23), 227-233,

Tolan, N., Genes, L.I., Spence, D.M. Merging microfluidics with microtitre technology for more efficient drug discovery. JALA 2008, 13(5), 275-279,


Carroll, J.S., Ku,C., Karunarathne,W., Spence, D.M. Red blood cell stimulation of platelet nitric oxide production indicated by quantitative monitoring of the communication between cells in the bloodstream. Anal. Chem. 2007, 79(14), 5133-5138,

Genes, L.I., Tolan, N.V., Hulvey, M.K., Martin, R.S., Spence, D.M. Addressing a vascular endothelium array with blood components using underlying microfluidic channels. Lab Chip 2007, 7, 1256-1259,

Hulvey, M.K., Genes, L.I., Spence, D.M., Martin, R.S. Fabrication and evaluation of a 3-dimensional microchip device where carbon microelectrodes individually address channels in the separate fluidic layers. Analyst 2007, 132, 1246-1253,

Ku, C., Karunarathne, W., Kenyon, S., Root, P., Spence, D. Fluorescence determination of nitric oxide production in stimulated and activated platelets. Anal. Chem. 2007, 79(6), 2421-2426,


Carroll, J., Raththagala, M., Subasinghe, W., Baguzi, S., D’amico Oblack, T., Root, P., Spence, D. An altered oxidant defense system in red blood cells affects their ability to release nitric oxide-stimulating ATP. Mol. BioSyst. 2006, 2, 305-311,

D’Amico Oblak, T. Root, P., Spence, D.M. Fluorescence monitoring of ATP-stimulated, endothelium-derived nitric oxide production in channels of a poly(dimethylsiloxane)-based microfluidic device. Anal. Chem. 2006, 78(9), 3193-3197,

Martin, R.S., Root, P.D., Spence, D.M. Microfluidic technologies as platforms for performing quantitative cellular analyses in an in vitro environment. Analyst 2006, 131, 1197-1206,

Price, A.K., Martin, R.S., Spence, D.M. Monitoring erythrocytes in a microchip channel that narrows uniformly: towards an improved microfluidic-based mimic of the microcirculation. J. Chromatogr. A 2006, 1111(2), 220-227,

Raththagala, M., Root, P.D., Spence, D.M. Dynamic monitoring of glutathione in erythrocytes, without a separation step, in the presence of oxidant insult. Anal. Chem. 2006, 78(24), 8556-8560,


Li, M.W., Spence, D.W., Martin, R.S. A microchip-based system for immobilizing PC 12 cells and amperometrically detecting catecholamines released after stimulation with calcium. Electroanalysis 2005, 17(13), 1171-1180,

Spence, D.M. Automation and microfluidic assays: in vitro models of the mammalian microcirculation. JALA 2005,10(4), 270-275,


Kovarik, M.L., Torrence, N.J., Spence, D.M., Martin, R.S. Fabrication of carbon microelectrodes with a micromolding technique and their use in microchip-based flow analyses. Analyst, 2004, 129, 400-405,

Price, A.K., Fischer, D.J., Martin, R.S., Spence, D.M. Deformation-induced release of ATP from erythrocytes in poly(dimethylsiloxane)-based microchip with channels that mimic resistance vessels. Anal. Chem. 2004, 76(16), 4849-4855,

Spence, D.M., Torrence, N.J., Kovarik, M.L., Martin, R.S. Amperometric determination of nitric oxide derived from pulmonary artery endothelial cells immobilized in a microchip channel. Analyst 2004, 129, 995-1000,

Spence, D.M. Bioanalytical challenges for analytical chemists. Analyst 2004, 129, 102-104,


Fischer, D.J., Torrence, NJ., Sprung, R.J., Spence, D.M. Determination of erythrocyte deformability and its correlation to cellular ATP release using microbore tubing with diameters that approximate resistance vessels in vivo. Analyst 2003, 128, 1163-1168,

Gordito, M.P., Kotsis, D., Minteer, S.D., Spence, D.M. Flow-based amperometric detection of dopamine in an immobilized cell reactor. J. Neurosci. Methods 2003, 124(2), 129-134,

Kotsis, D., Spence, D. A quantitative examination of some analytes involved in the control of pulmonary resistance by employing various microflow techniques and novel bio-reactors. Rev. Anal. Chem. 2003, 22(4), 235-253,

Kotsis, D.H., Spence, D.M. Detection of ATP-induced nitric oxide in a biomimetic circulatory vessel containing an immobilized endothelium. Anal. Chem. 2003, 75(1), 145-151,

Sprague, R.S., Olearczyk, J.J., Spence, D.M., Stephenson, A.H., Sprung, R.W., Longigro, A.J. Extracellular ATP signaling in the rabbit lung: erythrocytes as determinants of vascular resistance. Am. J. Physiol. Heart Circ. Physiol. 2003, 285(2), H693-H700,


Sprung, R., Sprague, R., Spence, D. Determination of ATP release from erythrocytes using microbore tubing as a model of resistance vessels in vivo. Anal. Chem. 2002, 74(10), 2274-2278,


Edwards, J., Sprung, R., Sprague, R., Spence, D. Chemiluminescence detection of ATP release from red blood cells upon passage through microbore tubing. Analyst 2001, 126, 1257-1260,


Edwards, J., Patel, A., Spence, D.M. Performance enhancement in flow reversal flow injection using on-capillary detection. Anal. Chim. Acta 2000, 417(2), 185-190,